Coated vesicles: characterization, selective dissociation, and reassembly.
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چکیده
منابع مشابه
Assembly polypeptides from coated vesicles mediate reassembly of unique clathrin coats
A protein activity has been identified in extracts of coated vesicles that enables purified clathrin triskelions to reassemble in vitro into coat structures of uniform size. Coats formed in the presence of this preparation, regardless of the buffer system employed, are uniform in size with a mean diameter of 78 nm (+/- 5 nm SD) and a sedimentation coefficient (S20,w) of approximately 250S. Anal...
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We have applied agarose gel electrophoresis as a novel step in the purification of clathrincoated vesicles . Preparations of coated vesicles obtained by sedimentation velocity and isopycnic centrifugation are resolved into two distinct fractions upon electrophoresis . The slower migrating fraction contains smooth vesicles, whereas the faster contains only coated vesicles and empty clathrin coat...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.9.4394